She and her owner were on their way to Philadelphia when Ellie went into labor.
The Tampa Fire Department also showed up to assist and all eight puppies and mom are now doing well.
Tampa Fire Rescue shared the sweet moments over social media.
The Tampa Bay Times reports 2-year-old golden retriever Eleanor Rigby- Ellie for short, went into labor at Gate F-81.
Dressed in denim capri trousers and a Hawaiian-print shirt, the owner snuggled right up to Ellie to comfort her through the process.
Service dog gives birth to 8 puppies at Florida airport gate
Plastic and cotton mats were placed on the ground, to give Ellie a sterile surface and protect the carpeting in the terminal.
"The unusual scene drew quite a crowd outside of the Gasparilla Bar at TIA's Airside F. Officers, airport employees, and passengers all paused to watch the pups" arrival.
Both Eleanor Rigby and Golden Nugget are service dogs to Van Atter, who suffers from mobility and pain issues, an airport spokeswoman told the publication. They were aware that Ellie was pregnant, but didn't realize how far along she was.
"Tampa paramedics do it all!"
Eight puppies were born in total.More news: PayPal is buying payments start-up iZettle for $2.2 billion ahead of IPO